Covalent targeting of PSMD14 by Eupalinolide B induces oncoprotein degradation and apoptosis in acute promyelocytic leukemia cells | Synapse
February 6, 2026Open Access
Covalent targeting of PSMD14 by Eupalinolide B induces oncoprotein degradation and apoptosis in acute promyelocytic leukemia cells
Key Points
The aim is to explore how Eupalinolide B impacts PSMD14 and affects oncoprotein levels in acute promyelocytic leukemia cells.
Utilized Eupalinolide B to target PSMD14 covalently
Assessed deubiquitinase activity
Measured levels of oncoproteins AKT1 and CDK4
Evaluated cell cycle progression and apoptosis rates
PSMD14 was successfully inactivated by Eupalinolide B
AKT1 and CDK4 levels decreased significantly
Cell cycle arrest occurred following treatment
Apoptosis was induced in leukemia cells as a result of cascaded effects
Abstract
Eupalinolide B covalently targets PSMD14, inhibiting its deubiquitinase activity. This leads to degradation of oncoproteins AKT1 and CDK4, resulting in cell cycle arrest and apoptosis in leukemia cells.