Comparative performance of four immunoassays for anti-laminin 332 serum IgG in mucous membrane pemphigoid: a multicentre prospective blinded study

Key Points

• This study aims to compare the effectiveness of four immunoassays for detecting anti-laminin 332 IgG in mucous membrane pemphigoid.
• Multicentre study design
• Use of four different immunoassays
• Blinded comparison of assay performance
• Assessment of diagnostic accuracy and specificity
• The keratinocyte footprint assay demonstrated the highest diagnostic accuracy among the assays tested.
• The laminin 332 Biochip® exhibited excellent specificity for diagnosis.
• Differences in performance metrics suggest varying utility of assays in clinical practice.