Diagnostic assessment of Hirschsprung disease using fluorescence confocal microscopy: A feasibility study

Key Points

• Fluorescence confocal microscopy shows comparable diagnostic accuracy to frozen sections in identifying Hirschsprung disease.
• Tissue quality is well-preserved as unfixed specimens are utilized during fluorescence confocal microscopy.
• This method proves beneficial where local access to expert pathologists is limited, potentially improving diagnoses.
• The study highlights an easy-to-implement approach for assessing affected bowel in Hirschsprung disease.