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March 3, 2026
Unlocking the complexity of macromolecular protein heterogeneity of glycoprotein IgE via an optimized online digestion middle-down proteomics approach
RL
Ruijie Liu
Sun Yat-sen University
ZL
Zixin Liang
SX
Shujun Xia
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Key Points
Glycoprotein IgE heterogeneity was successfully analyzed, shedding light on its complex structure.
In findings, 12 distinct glycoforms were identified using an optimized online digestion approach.
Analysis involved a middle-down proteomics strategy, enhancing protein characterization and understanding.
Highlights the potential for deeper insights into glycoproteins, paving the way for further studies on immune responses.
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Liu et al. (Sat,) studied this question.
synapsesocial.com/papers/69a75ef4c6e9836116a29fcb
https://doi.org/https://doi.org/10.1016/j.aca.2026.345161
Unlocking the complexity of macromolecular protein heterogeneity of glycoprotein IgE via an optimized online digestion middle-down proteomics approach | Synapse