March 3, 2026

Preparation of Agrobacterium Culture for Gene Delivery into Plant Cells

Key Points

Gene delivery into plant cells is achievable using prepared Agrobacterium cultures.
Acetosyringone induces virulence gene expression, enhancing the transformation process.
The procedure involves washing and resuspending the culture to ensure antibiotic removal.
Efficient gene transfer to plant tissues may enable advances in agricultural biotechnology.

Abstract

Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.

Connected Papers

Building similarity graph...

Analyzing shared references across papers

Discussion

Cite this study

TODO TODO (Mon,) studied this question.

www.synapsesocial.com/papers/69a765e4badf0bb9e87dadff — DOI: https://doi.org/10.3791/24361

Preparation of Agrobacterium Culture for Gene Delivery into Plant Cells

Key Points

Abstract

Citation Network

Connected Papers

Discussion

Cite this study

Authors

Journals

Actions

References and Citations

Citation Network

Connected Papers

Discussion