Abstract 3399: Highly multiplexed imaging allows for spatial characterization of tumor organoid models

Key Points

• To evaluate the effectiveness of highly multiplexed imaging in characterizing tumor organoid models and their structural representation.
• Produced patient-derived lymphoma and glioblastoma organoids.
• Fixed and embedded organoids in hydroxyethyl agarose gel and paraffin.
• Utilized automated sequential immunofluorescence imaging for detecting 80 proteins with a multiplex panel.
• Selected 50 markers for organoid profiling targeting cell phenotypes and metabolic states.
• Lymphoma organoid showed cell type frequencies of T-cell subsets and B-cells matching flow cytometric analysis.
• GBM organoid contained various neural cell states akin to single-plex assays.
• Proliferative T-cells in lymphoma organoid exhibited higher glycolytic and mitochondrial enzyme expression compared to non-proliferative subsets.

Abstract