Pre-exposure to chilling stress enhances proline-mediated cold tolerance in barley seedling roots via metabolic modulation

Key Points

• Enhanced cold tolerance was observed in barley seedlings after pre-exposure to chilling stress, indicating proline's key role.
• The cold tolerance improved significantly, with a 30% increase in root resilience following proline treatment under stress conditions.
• Observational analysis focused on metabolic modulation processes in barley roots under chilling stress conditions.
• Findings highlight the importance of proline in preparing barley seedlings for cold stress, indicating potential agricultural benefits.