Challenges in mRNA-based time since deposition estimation: A multiplex MPS primer panel investigation

Key Points

• Estimation accuracy of mRNA deposition remains challenging, impacting biomarker interpretation.
• The study reveals significant variability in mRNA levels over time, potentially confounding results.
• Assessment using multiplex mps primer panels highlights the need for standardization in methodologies.
• Further validation is necessary to establish reliable mRNA deposition estimates in diverse conditions.