Smartphone-Assisted Urinary Methylmalonic Acid Sensing by an Imidazole-Based Probe via Analyte-Triggered Aggregation Mechanism

Key Points

• The study aims to develop a smartphone-assisted method to sense methylmalonic acid, a biomarker for vitamin B12 deficiency, using an imidazole-based probe.
• Developed an imidazole-based fluorescent probe for methylmalonic acid.
• Investigated the analyte-triggered aggregation mechanism for fluorescence enhancement.
• Utilized smartphone technology for real-time sensing of methylmalonic acid.
• Achieved effective fluorescence response upon exposure to methylmalonic acid.
• Demonstrated potential for a turn-on-based sensing mechanism with high efficiency.