Multicolor STED Imaging of Cells and Extracellular Vesicles using Xanthene-conjugated Polymer Dots

Key Points

• To improve STED imaging using high-quality fluorescent probes for better visualization of cells and extracellular vesicles.
• Utilized xanthene-conjugated polymer dots as fluorescent probes
• Employed multichannel STED nanoscopy for imaging
• Focused on comparing imaging performance with traditional probes
• Demonstrated enhanced resolution in imaging cellular structures
• Showed improved detection of extracellular vesicles
• Indicated the effectiveness of xanthene-conjugated polymers in multicolor imaging