Parvimonas oralis sp. nov. and Parvimonas pharyngis sp. nov., isolated from the pharyngeal swabs of coal miners

Four Gram-stain-positive, catalase-negative, oxidase-negative, strictly anaerobic, non-spore-forming, non-motile, coccoid bacteria (G1425T/G1967 and G1604T/G1641) were isolated from the pharyngeal swabs of coal miners in Shanxi Province of China. Phylogenetic analysis of the 16S rRNA gene and 194 core genes revealed that these 4 strains belong to the genus Parvimonas. These strains were most closely related to Parvimonas micra ATCC 33270T and Parvimonas parva S3374T. Average nucleotide identity (84.0-90.9%) and digital DNA-DNA hybridization values (27.3-42.0%) were below the species-level thresholds. The major fatty acids of all four strains were C16:0, C18:1 ω9c and C18:0. Major polar lipids were composed of diphosphatidylglycerol and phosphatidylethanolamine. Ubiquinone-8 was the sole ubiquinone detected in strains G1425ᵀ and G1604ᵀ. Based on phenotypic and phylogenetic evidence, we propose that strains G1425T and G1604T represent two novel species of the genus Parvimonas, respectively, with the names Parvimonas oralis sp. nov. and Parvimonas pharyngis sp. nov. The type strains are G1425T (=GDMCC 1.5468T=JCM 37774T) and G1604T (=GDMCC1.5469T=JCM 37775T).