Evaluating metagenomic sequencing as a stool-based diagnostic in children with presumptive TB in Uganda
Key Points
Metagenomic sequencing did not enhance sensitivity for TB detection.
Novel diagnostic targets were identified for potential molecular testing improvements.
Stool DNA analysis presents a non-invasive option for TB assessment in children.
Enhancing TB diagnostics can lead to better health outcomes in resource-limited settings.
Abstract
Metagenomic sequencing of stool DNA did not increase sensitivity of TB detection, but identified novel targets for molecular testing that may support development of more sensitive tests.