Identification of the Novel HLA‐A*02:1040:02 Allele by Next‐Generation Sequencing

Key Points

• The research aims to identify and characterize the HLA-A*02:1040:02 allele through advanced sequencing methods.
• Utilized next-generation sequencing for precise genetic analysis.
• Focused on the nucleotide substitutions in HLA gene alleles.
• Analyzed differences at codon 283 in exon 5.
• Identified HLA-A*02:1040:02 as distinct from HLA-A*02:01:01:01.
• Confirmed the difference is due to a single nucleotide change (CCC>TCC).