June 8, 2020STAR Protocols25 citationsOpen Access
Protocol for Single-Nucleus Transcriptomics of Diploid and Tetraploid Cardiomyocytes in Murine Hearts
Structured PICO
P
Population
Murine hearts (diploid and tetraploid cardiomyocytes)
I
Intervention
Single-nucleus RNA-sequencing applied to fluorescence-activated cell sorting-selected diploid and tetraploid cardiomyocytes
O
Outcome
Characterization of heterogeneity and molecular distinctions between diploid and tetraploid cardiomyocytes
This paper provides a protocol for using single-nucleus RNA-sequencing to characterize the molecular differences between diploid and tetraploid murine cardiomyocytes.
Abstract
Murine cardiomyocytes undergo proliferation, multinucleation, and polyploidization during the first 3 weeks of postnatal life, resulting in a mixture of diploid and tetraploid cardiomyocytes in the heart. Understanding the molecular differences between diploid and tetraploid cardiomyocytes from these processes has been limited due to lack of unique markers and their heterogenous origins. Here, we apply single-nucleus RNA-sequencing to fluorescence-activated cell sorting-selected diploid and tetraploid cardiomyocytes to characterize their heterogeneity and molecular distinctions. For complete details on the use and execution of this protocol, please refer to Cui et al. (2020).