Euphorbia bicolor Xylene Extract Induces Mitochondrial and Endoplasmic Reticulum Stress-Mediated Apoptotic Pathways in MDA-MB-231 and T47D Cells

Key Points

• This research aims to explore the effects of Euphorbia bicolor xylene extract on breast cancer cell viability and the underlying mechanisms.
• Evaluated antiproliferative activities in T47D and MDA-MB-231 cells
• Assessed apoptosis through calcium influx and reactive oxygen species generation
• Used specific inhibitors like capsazepine and NAC to test pathways
• Monitored protein expression related to apoptotic pathways
• E. bicolor xylene extract reduced cell viability in a dose-dependent manner
• Induced intracellular calcium overload and apoptosis in MDA-MB-231 cells
• Generated 10-fold higher ROS in T47D cells compared to MDA-MB-231
• Activated various apoptotic pathways as shown by caspase activation
• Downregulated PI3K/Akt signaling pathway in both cell lines

Abstract