From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat

Key Points

• The research aims to establish an efficient transformation platform for Tartary buckwheat using elite germplasm.
• Identified elite germplasm G253 with high callus induction and proliferation.
• Established an Agrobacterium-mediated transformation system for creating transgenic plants.
• Developed a protoplast transient transformation method from morphogenic callus.
• G253 demonstrated superior morphogenic callus induction and growth compared to other germplasms.
• The Agrobacterium system successfully generated stable transgenic plants.
• The protoplast transformation system was found to be efficient, enhancing transformation speed.