High-throughput RNA sequencing of AML12 cells

Key Points

• The study aims to analyze gene expression changes in AML12 cells following the stable knockdown of Drosha and Dicer.
• Implemented high-throughput RNA sequencing to evaluate gene expression
• Knocked down Drosha and Dicer in AML12 cells
• Stable knockdown methodology to ensure consistent results
• Identified specific gene expression alterations due to Drosha knockdown
• Observed different expression profiles in Dicer knockdown compared to controls
• Findings suggest distinct roles of Drosha and Dicer in regulating gene expression

Abstract