Abstract 721: High-plex 3D imaging of the tumor-immune microenvironment.

Abstract Solid tumors evolve in competition with the immune system and must attract resources such as nutrients and oxygen to proliferate and invade normal tissue. This results in complex spatial distribution of tumor, immune, and stromal cell types within the tumor microenvironment (TME). Regulatory interactions among these cells involved in tumor progression and response to therapy are found on a wide range of length scales from subcellular membrane domains (involved in autocrine signaling between PD1 and PDL1 for example) to extended structures spanning thousands of cell diameters such as lymphoid aggregates and tumor invasive boundaries. Highly multiplexed tissue imaging (spatial proteomics), in combination with other single cell methods, promises to reveal the composition and regulation of these TME features in molecular detail but the great majority of data collected to date, including all classical histopathology based on hematoxylin and eosin (H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 721.