Integrated histomolecular diagnosis of mesenteric anisakiasis

Key Points

• The aim is to present an innovative diagnostic approach for mesenteric anisakiasis using histomolecular techniques.
• Clinical examination of gastrointestinal symptoms in a patient.
• Imaging conducted to assess duodeno-ileitis.
• Resection of an ileal segment performed due to mesenteric ischaemia.
• Combination of histopathological analysis and shotgun metagenomic analysis to identify Anisakis spp. larva.
• Identification of pre-adult Anisakis spp. larva in a mesenteric nodule.
• Successful application of integrated diagnostic methods to enhance detection accuracy.
• Complications included mesenteric ischaemia requiring surgical intervention.

Abstract