Cascade Signal Amplification-Based Fluorescent Biosensor Utilizing Exonuclease III and Self-Locking DNAzyme Synergy for microRNA Detection

Key Points

• The biosensor achieved high sensitivity for microRNA detection, crucial for early disease diagnosis.
• Utilizing a novel synergy of exonuclease III and DNAzyme, the method demonstrated reliable performance in identifying specific miRNAs.
• Signal amplification techniques were effectively employed to reduce background interference in the detection process.
• These findings point to promising applications in the diagnosis of cancer and other critical diseases.