Microbacterium salitolerans sp. nov., isolated from saline soil

Strain G2-8 T was isolated from a soil sample collected from the saline area of Baodi District in Tianjin City. The isolate was a Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, and its colonies were round, raised, pale yellow, with a smooth surface, regular and clean edges. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain G2-8 T belonged to the genus Microbacterium and shared the highest sequence similarity with Microbacterium excoecariae CBS5P-1 T (98.5%), followed by Microbacterium amylolyticum N5 T (98.2%), Microbacterium gubbeenense DSM 15944 T (98.1%), Microbacterium indicum BBH6 T (97.5%), Microbacterium suaedae YZYP 306 T (97.5%) and Microbacterium karelineae TRM 80801 T (97.4%). Phylogenomic analysis showed that strain G2-8 T was related to members of the genus Microbacterium and represented an independent lineage. Growth occurred at 4–40 °C (optimum, 28 °C), up to 13.0% (w/v) NaCl (optimum, 0–2.0%) and pH 6.0–10.0 (optimum, pH 8.0). Its main fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The major respiratory quinone of strain G2-8 T was MK-10, MK-11 and MK-12. The polar lipid profile of strain G2-8 T comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified polar lipids. The genome of strain G2-8 T was 3.46 Mbp with a G+C content of 69.7 mol%. The average nucleotide identity (ANI) values between strain G2-8 T and other related species of the genus Microbacterium were found to be low (ANIm <84.8%, ANIb <77.2% and OrthoANIu <79.6%). Furthermore, the digital DNA–DNA hybridization and average amino acid identity values between strain G2-8 T and the closely related species ranged from 19.0 to 24.2% and from 65.9 to 79.6%, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain G2-8 T represents a novel species of the genus Microbacterium , for which the name Microbacterium salitolerans sp. nov. is proposed. The type strain is G2-8 T (=KCTC 49757 T =GDMCC 1.2353 T ).