Self‐aggregation processes are ubiquitous in living systems and play vital roles in maintaining normal physiological functions. Inspired by these natural phenomena, constructing artificial aggregates within cells is of great significance for understanding the complex mechanisms of life activities and precisely regulating cellular processes. Owing to its excellent programmability and stimulus responsiveness, DNA nanotechnology offers unprecedented opportunities for building intelligent nanodevices inside living cells, far more dynamically regulable than traditional materials. This review systematically summarizes recent strategies for achieving intelligent intracellular aggregation of DNA nanostructures in response to various stimuli, including protons, metal ions, enzymes, nucleic acids, and sequential stimuli. Furthermore, we discuss the specific applications of these DNA‐based smart aggregates, including in situ biosensing and cellular function regulation. Finally, we provide an outlook on key challenges and prospects in this emerging field.
Shi et al. (Thu,) studied this question.