Theoretical investigation into the position-dependent influence of l -deoxynucleotides in the template strand on T7 RNA polymerase transcription activity

Key Points

• This research aims to explore how the position of l-deoxynucleotides in the template strand affects T7 RNA polymerase transcription activity.
• Conducted molecular dynamics simulations
• Analyzed structural attributes of DNA–RNA hybrids
• Investigated the impact of l-deoxynucleotides at various positions
• Identified position-dependent effects on transcription activity
• Uncovered structural changes in DNA–RNA hybrids correlated with transcription efficiency