Ratiometric Near-Infrared Fluorescence Probes for Quantitative Monitoring of Mitochondrial pH Dynamics in Drosophila Melanogaster and HeLa Cells during Metformin Treatment

Key Points

• The aim is to develop fluorescent probes for tracking mitochondrial pH changes during metformin treatment.
• Synthesis of two ratiometric fluorescent probes, AH⁺ and BH⁺.
• Targeting of probes to mitochondria in Drosophila melanogaster and HeLa cells.
• Quantitative analysis of pH dynamics under starvation and oxidative stress conditions.
• The probes effectively monitored pH changes in mitochondria during treatment.
• Significant pH variations were observed under metformin influence.
• Demonstrated probe selectivity and sensitivity in live cellular environments.