ABSTRACT High consumption of colorful fruits and vegetables correlates with low dementia risk, but the exact molecules and the underlying biological mechanisms governing their bioactive profiles are largely unknown. Using a 10‐year observational cohort study coupled with an AI‐driven systems pharmacology platform, we identified a natural triterpenoid compound found in colorful fruits and vegetables, α‐Amyrin (αA), as a therapeutic candidate for Alzheimer's disease (AD). The efficacy of αA in treating the symptoms of AD, such as Tau tangles, damaged mitochondria, and memory loss, was examined using cross‐species models; αA retained memory in AD‐like animal models while also strongly inhibiting Tau pathology, especially p‐Tau217, in a cellular ‘Tau seeding’ system and in TauP301S mice, followed by validation using a human 3D microfluidic system. At molecular level, αA is a robust mitochondrial regulator, enhancing mitochondrial stress resilience and activation of mitophagy. Mechanistically, αA inhibits dual leucine zipper kinase (DLK), leading to the inhibition of DLK‐Sterile Alpha and TIR Motif Containing 1 (SARM1)‐dependent neurodegeneration; this inhibition frees unc‐51 Like Autophagy Activating Kinase 1 (ULK1) from the ULK1‐SARM1 complex, allowing it to participate in autophagy/mitophagy. αA also shows strong translational potential with a 10.1 h half‐life and the ability to cross the blood‐brain barrier. Our results indicate that αA may act as a mitochondrial guardian against AD via modulating the DLK‐SARM1‐ULK1‐autophagy/mitophagy axis while further preclinical and clinical studies are warranted.
Cao et al. (Thu,) studied this question.