ABSTRACT Nanozymes are powerful alternatives to natural enzymes, yet the sustainable design of hydrolase nanozymes using renewable ligands remains underexplored. Lignin, as an abundant aromatic biopolymer, offers a promising and green ligand for constructing sustainable nanozymes. In this work, a Ce‐doped lignin‐based hydrolase nanozyme (Ce‐AL) is synthesized using Ce ions coordinated with aminated industrial lignin. The resulting Ce‐N x centered Ce‐AL enable the hydrolysis of phosphate esters and even stable protein amide bonds. Notably, the hydrolase‐like activity of Ce‐AL at 100°C is 2.03 times that at 40°C, attributable to the thermally optimized Ce‐N x sites within the protective lignin scaffold. The Ce‐AL effectively combats stubborn biofilms formed by Gram‐negative ( Escherichia coli ) and Gram‐positive ( Staphylococcus aureus ) bacteria by selectively hydrolyzing key proteinaceous and nucleotide components of the extracellular polymeric substances and bacterial cell walls. This work highlights lignin as a renewable platform for designing advanced, stimuli‐responsive nanozymes.
Liu et al. (Fri,) studied this question.