Infected with virulent DHAV-3, ducklings showed 2,355 differentially expressed genes in liver tissue compared to 322 DEGs in those immunized with the attenuated strain HB80 at 2 days post-infection.
Infection with virulent DHAV-3 induces a massive transcriptomic shift and robust activation of immune pathways in duck livers compared to the attenuated vaccine strain, highlighting the molecular basis of its virulence.
Effect estimate: null (95% CI null)
Absolute Event Rate: 2355% vs 322%
p-value: p=null
Introduction Duck hepatitis A virus type 3 (DHAV-3) causes acute fatal hepatitis in ducklings. Methods This study compared host transcriptomic responses in duck livers following infection with virulent (HB) or attenuated (HB80) strains. Results RNA sequencing (RNA-Seq) revealed that the virulent HB strain induced 2,355 differentially expressed genes (DEGs) at 2 days post-infection (dpi), compared to only 322 DEGs triggered by the attenuated HB80 strain. Functional analysis showed that the HB strain robustly activated immune pathways, particularly Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling, leading to a potent type I interferon (IFN-I) response and marked chemokine upregulation. In contrast, the HB80 strain elicited a markedly milder immune reaction. Among the DEGs, 77 immune-related genes were identified, with significant enrichment in the IFN-I signaling pathway, suggesting their critical role in initiating an interferon storm and subsequent chemokine upregulation. Selected key genes (IFN-α2, RSAD2, RIG-I, MDA5, TBK1, TLR7) were validated by RT-qPCR and ELISA for targeted protein confirmation. Discussion Our study findings suggest that paternal care is associated with medical or co-medical professional choices. Further prospective studies are required to determine causal relationships and investigate other factors related to such choices, given the non-association of all other variables in the study.
Fu et al. (Wed,) conducted a other in Duck hepatitis A virus type 3 (DHAV-3) infection (n=150). Virulent DHAV-3 strain (HB) and attenuated DHAV-3 strain (HB80) vs. Physiological saline was evaluated on Differentially expressed genes (DEGs) in duck livers post-infection (null, 95% CI null, p=null). Infected with virulent DHAV-3, ducklings showed 2,355 differentially expressed genes in liver tissue compared to 322 DEGs in those immunized with the attenuated strain HB80 at 2 days post-infection.