Comparative Evaluation of SARS-CoV-2 Antigens as Capture and Detection Elements in an In-House Antigen-Based ELISA for COVID-19 Total Antibody Detection

Key Points

• The study aims to evaluate the effectiveness of SARS-CoV-2 antigens in detecting total antibodies using an in-house ELISA.
• Used RBD-based enzyme immunoassay (EIA) for detection
• Tested antibody responses in both human and animal samples
• Assessed efficiency and reliability for serological surveillance
• RBD-based EIA provides reliable detection of total antibodies
• The platform is effective for both human and animal testing
• Supports applications in vaccine evaluation and One Health monitoring