Strain DSM 117947 T was isolated from a Micromonospora matsumotoense co-culture originating from a water sample collected in Germany. The strain was subjected to a polyphasic taxonomic analysis. It exhibited 99. 3% 16S rRNA gene sequence similarity with Nocardioides aurantiacus DSM 12652 T. Digital DNA-DNA hybridization and average nucleotide identity values between the strain and its close phylogenetic neighbour were below the threshold of 70% and 95−96% for prokaryotic species demarcation, respectively. The strain had a polar lipid profile composed of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmethylethanolamine (PME), phosphatidylinositol (PI), glycophospholipid (GPL), and phospholipids (PLs). The predominant menaquinone (>20%) was MK-8 (H 4). The major fatty acids (>5%) were C 16. 0, C 16: 1 ω7c, and C 18: 1 ω9c. The genomic G + C content of the strain is 73%. The chemotaxonomic, biochemical, enzymatic, and genomic features distinguished the strain from its close relative, and justify its assignment to a novel species, for which the name Nocardioides aquaegermanicae sp. nov. , is proposed, with strain DSM 117947 T (WGₒrange T = KCTC 59414 T) as the type strain.
Zhou et al. (Tue,) studied this question.