Antibody-mediated immune responses in mucosal tissues are critical for defending against pathogens while maintaining homeostasis with commensals. Nasal vaccination aims to induce local protection in the upper airway mucosa. Although B cell–driven immunity is well characterized in gut-associated lymphoid tissues such as Peyer’s patches and mesenteric LNs, much less is known about analogous processes in the upper airways. Here, we show that B cell receptor (BCR) affinity and CCR6 regulate germinal center (GC) seeding and class-switch recombination (CSR) to IgA in nasal-associated lymphoid tissue (NALT) following nasal vaccination. B cells bearing low-affinity BCRs failed to upregulate CCR6 and did not support T follicular helper cell differentiation or seed GCs in the NALT. CCR6-deficient B cells were unable to migrate to the NALT subepithelial dome or undergo IgA CSR and seed GC effectively in response to nasal vaccination or commensal bacteria signals. Thus, effective targeting of B cell clones to induce CCR6 expression is essential for nasal vaccine design.
Liu et al. (Wed,) studied this question.