Investigation of the hemostatic efficacy and acute histopathological effects of herbal algan hemostatic agent in partial nephrectomy model in rats

Abstract Background Uncontrolled bleeding during partial nephrectomy poses a significant surgical challenge. This study evaluates the hemostatic efficacy and acute histopathological effects of Algan Hemostatic Agent (AHA), a novel plant-based agent, in a rat model of partial nephrectomy. Methods Twenty-four rats underwent partial nephrectomy and were randomized to three groups: control (saline), AHA powder, and AHA liquid-impregnated sponge. Hemostasis was attempted for up to 3 applications, and success rates were compared. Kidney tissue was harvested for histopathological evaluation, assessing tissue necrosis, coagulation, hemorrhage, and inflammation. Results AHA powder achieved hemostasis in all 8 rats (100%) after a single application, which was significantly higher than the control group ( p < 0.001). The AHA liquid-impregnated sponge achieved hemostasis in 6 out of 8 rats (75%) after the first application and in all 8 rats (100%) after the second. No significant histopathological damage was observed in any group. Conclusion AHA, particularly in powder form, demonstrates promising hemostatic efficacy during partial nephrectomy in rats with no significant acute histopathological damage. Further research is warranted to evaluate long-term effects and compare AHA with existing hemostatic agents