A GC-MS method in selected ion monitoring (SIM) mode was optimized and validated for the determination of twelve polycyclic aromatic hydrocarbons (PAHs) in Barbus carpathicus tissues (muscle and hepatopancreas). Two chromatographic columns (HP-5ms and DB-EUPAH) were evaluated with multiple temperature programs, and the DB-EUPAH column proved superior for separating critical PAH isomers within a 25-minute runtime. Method validation demonstrated excellent linearity (R2 ≥ 0.995), with deviation from linearity B.carpathicus tissue analysis. The developed method provides a reliable, validated analytical tool suitable for routine monitoring and risk assessment of PAH contamination in B.carpathicus, supporting environmental monitoring and food safety protocols.
Lakatošová et al. (Fri,) studied this question.