Rapid and colorimetric assay for detection of S. pneumoniae based on hydrogen peroxide release and analysis using color image processing

Key Points

• The aim is to develop a fast and simple assay for detecting Streptococcus pneumoniae, addressing limitations of existing methods.
• Developed a colorimetric assay based on hydrogen peroxide release.
• Used image processing techniques to analyze color changes.
• Evaluated the assay against existing clinical methods.
• The new assay produced results more rapidly than conventional techniques.
• Colorimetric changes successfully indicated S. pneumoniae presence.
• Analysis showed high sensitivity and specificity.