Antinuclear antibody targets in autoimmune hepatitis and drug-induced liver injury: Diagnostic relevance of nucleosome antibodies.

Key Points

• This study investigates the antigen specificity of antinuclear antibodies in autoimmune hepatitis and drug-induced liver injury, focusing on their diagnostic relevance.
• Retrospective, cross-sectional analysis of ANA-positive patients with autoimmune hepatitis or drug-induced liver injury.
• ANA patterns determined by indirect immunofluorescence.
• Specific antigen characterization using ELISA.
• Out of 81 patients, a homogeneous ANA pattern was more common in autoimmune hepatitis (75.9%) than in drug-induced liver injury (48.1%).
• Anti-nucleosome antibodies were found in 51.2% of AIH patients and 15.4% of DILI patients.
• Anti-nucleosome antibodies exhibited a specificity of 84.6% and a sensitivity of 51.2%.
• Area under the curve (AUROC) for anti-nucleosome antibodies was 0.85, indicating high diagnostic accuracy.