Characterisation of the Novel HLA‐B*27:301 Allele by Sequencing‐Based Typing

Key Points

• The aim is to characterize the HLA-B*27:301 allele and identify differences from HLA-B*27:05:02:01.
• Utilized sequencing-based typing techniques
• Examined nucleotide variations
• Focused on codon 333 in exon 7
• Identified one nucleotide substitution at codon 333
• Confirmed genetic difference from HLA-B*27:05:02:01