Synthesis and characterization of ZrFe 2 O 4 and ZrFe 2 O 4 @UiO-66-NH 2 nanoparticles for efficient immobilization of Humicola insolens lipase: a comparative study of precipitation-crosslinking versus covalent binding methods

Key Points

• The aim is to compare the effectiveness of two methods for immobilizing lipase onto nanoparticles.
• Synthesize ZrFe2O4 and ZrFe2O4@UiO-66-NH2 nanoparticles.
• Apply precipitation-crosslinking and covalent binding methods for immobilization.
• Characterize synthesized nanoparticles and evaluate immobilization efficiency.
• Both methods successfully immobilized lipase onto nanoparticles.
• Covalent binding resulted in higher immobilization efficiency compared to precipitation-crosslinking.
• Nanoparticles retained lipase activity after immobilization.