Background: Platycladus orientalis L. is a drought-tolerant conifer valued for its ornamental and medicinal properties. However, efficient regeneration systems for this species remain limited, hindering its propagation and conservation. Aims: The aim of this study was to develop a reliable protocol for indirect organogenesis of Platycladus orientalis under in vitro conditions, evaluating the influence of explant type, culture medium, light exposure, and pretreatment on regeneration efficiency. Methods: Cotyledon, hypocotyl, and radicle explants were cultured on different media formulations. The effects of light and darkness during callus induction and shoot elongation were compared. Seeds underwent or avoided vernalization and scarification treatments to assess their influence on germination and callus formation. Results: Cotyledon explants achieved the highest callus induction rate, reaching 74.06%, particularly under dark conditions. Exposure to light during elongation significantly enhanced callus proliferation and shoots differentiation. Quoirin and LePoivre medium promoted the greatest number of adventitious shoots, with an average of 7.9 shoots per explant, while other media tested showed lower effectiveness. Germination was higher in non-vernalized and non-scarified seeds cultured in Quoirin and LePoivre medium. Conclusions: The established protocol enables efficient indirect organogenesis and shoot regeneration of Platycladus orientalis using cotyledon explants and Quoirin and LePoivre medium. The finding provides a valuable tool for clonal propagation and conservation of this species, supporting both ornamental cultivation and the preservation of its genetic resources.
Lucena-Soriano et al. (Fri,) studied this question.
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