What question did this study set out to answer?

To explore the relationship between vitamin D status, vitamin D receptor expression, and T-cell senescence in pediatric patients with atopic dermatitis.

February 26, 2026Open Access

The Hidden Pathway: Membrane Vitamin D Receptor Deficiency and T-Cell Senescence in Pediatric Atopic Dermatitis – A Retrospective and Cross-Sectional Investigation

Key Points

To explore the relationship between vitamin D status, vitamin D receptor expression, and T-cell senescence in pediatric patients with atopic dermatitis.
Analyzed serum 25-hydroxyvitamin D levels in 408 pediatric participants and controls.
Measured soluble vitamin D receptors (sVDR) in a subset of 89 patients using ELISA.
Assessed T-cell senescence markers and vitamin D receptor expression via flow cytometry.
Children with atopic dermatitis had significantly lower serum 25(OH)D and sVDR levels.
Expanded senescent T-cell populations were observed in various subsets including CD3⁺ and CD8⁺ cells.
Membrane VDR (mVDR) expression was significantly reduced in all immune cells of pediatric atopic dermatitis patients.

Abstract

Background: Pediatric Atopic dermatitis (AD) is a common, relapsing inflammatory skin disease in children. While linked to vitamin D deficiency, the roles of T-cell senescence and vitamin D receptor (VDR) signaling remain unclear. Objective: To explore vitamin D status, VDR expression (soluble/sVDR, membrane/mVDR, nuclear/nVDR), and T-cell senescence in pediatric AD. Methods: Serum 25-hydroxyvitamin D (25(OH)D) levels were analyzed in 408 pediatric AD patients and matched controls. In a subset (89 patients, 35 controls), plasma sVDR was measured by ELISA. T-cell immunophenotyping, senescence markers (CD27 − CD28 − ), and mVDR/nVDR expression were assessed via flow cytometry. Results: Children with AD had significantly lower serum 25(OH)D ( p < 0.05) and sVDR ( p < 0.05). Although major T-cell subset proportions were similar, children with AD showed expanded senescent populations within CD3⁺ ( p < 0.01), CD8⁺ ( p < 0.05), CD4⁺ ( p < 0.05), and Regulatory T (Treg) cells ( p < 0.01). mVDR expression was pervasively reduced across all immune cells in pediatric AD (for CD3-, CD3+ and CD8+: p< 0.0001; for CD4+, T follicular helper (Tfh) cells: p < 0.001; for Treg: p < 0.01). Senescent cells expressed higher mVDR than non-senescent cells in both groups (NC: CD3-, p < 0.0001; CD3+ and CD8+, p < 0.01; CD4+, p < 0.001. AD: CD3-, CD3+, CD8+ and CD4+, p < 0.0001), yet overall children with AD exhibited lower mVDR levels both in non-senescent and senescent cells (non-senescent cells: CD3-, p < 0.001; CD3+ and CD4-, p < 0.0001; CD4+, p < 0.01. senescent cells:CD3-, CD3+ and CD8+, p < 0.0001; CD4+, p < 0.01). nVDR alterations were more limited, with elevation only in Tfh cells ( p < 0.05) of children with AD. Conclusion: Pediatric AD is characterized by accelerated T-cell senescence and a widespread mVDR pathway deficiency. This receptor defect, coupled with vitamin D deficiency, may drive immune dysregulation and premature T-cell aging, highlighting potential therapeutic targets in both ligand and receptor pathways. Keywords: 25-hydroxyvitamin D, vitamin D receptor, T cell, senescence, pediatric atopic dermatitis

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Cite This Study

Zhu et al. (Sun,) studied this question.

synapsesocial.com/papers/699fe39d95ddcd3a253e7aea https://doi.org/https://doi.org/10.2147/jaa.s579390

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