Portable smartphone enabled colorimetric detection of tetracycline in commercial milk samples via peroxidase-mimicking ZnO–Pd nanobolts

Key Points

• The goal is to develop a portable and efficient method to detect tetracycline in commercial milk using nanozymes.
• Utilized ZnO–Pd nanobolts as a peroxidase-mimicking nanozyme
• Employed a turn-off assay approach where tetracycline competes with a chromogenic substrate
• Developed a smartphone-enabled detection system for colorimetric analysis
• Achieved a limit of detection (LOD) of 2.9 nM for tetracycline
• Demonstrated the capability to detect tetracycline in commercial milk samples effectively
• Circumvented traditional methods by providing a portable solution for antibiotic detection