Enhancing prebiotic production by engineering the levanbiose-binding site of Erwinia tasmaniensis levansucrase

Key Points

• The research aims to enhance the production of bioactive LFOS by modifying the levanbiose-binding site in levansucrase.
• Engineered the levanbiose-binding site in Erwinia tasmaniensis levansucrase.
• Assessed the impact on levan polymerization process.
• Evaluated yields of bioactive LFOS post-engineering.
• Engineering effectively blocks levan polymerization.
• Demonstrated high-yield production of bioactive LFOS.