Identification of Key Genes Associated With Epithelial Barrier Dysfunction by Comprehensive Analysis and Experimental Validation.

Key Points

• The aim is to identify genes linked to epithelial barrier dysfunction and validate their roles in barrier improvement.
• Conducted a comprehensive analysis of gene expression related to epithelial barriers.
• Performed experimental validation in airway epithelial cells, epidermal keratinocytes, and intestinal epithelial cells.
• Targeted specific genes (CDC7 and TCN1) to assess their impact on barrier function.
• Targeting CDC7 and TCN1 improved barrier function in all tested cell types.
• Demonstrated the potential of these genes as therapeutic targets for epithelial dysfunction.