Microbial Cell-Free DNA Sequencing as a Novel Diagnostic for Culture-Negative Gemella haemolysans Endocarditis

Key Points

• To explore the use of plasma microbial cell-free DNA (mcfDNA) sequencing for diagnosing culture-negative Gemella haemolysans endocarditis.
• Utilized plasma samples for sequencing
• Analyzed microbial cell-free DNA
• Assessed the ability to detect Gemella haemolysans
• Identified Gemella haemolysans in culture-negative cases
• Improved diagnosis accuracy using mcfDNA
• Enabled more targeted therapy options for patients