Affinity-maturation engineering via phage display to optimize anti-idiotype antibodies for neutralizing antibody assays.

Key Points

• The study aims to enhance the affinity of anti-idiotype antibodies for better neutralizing antibody assays.
• Used phage display for antibody engineering
• Focused on optimizing affinity of anti-idiotype antibodies
• Developed a competitive ligand-binding assay for validation
• Affinity-improved anti-IDs were successfully developed
• Created a robust and effective binding assay for neutralizing antibodies