Recent advances in protein synthesis: from chemical synthesis to backbone editing

Key Points

• This research addresses the need for precise control over protein structure and function in scientific applications.
• Review of solid-phase and solution-phase peptide synthesis methods.
• Discussion of unnatural amino acid incorporation techniques.
• Analysis of backbone editing reactions and their applications in protein engineering.
• Identification of limitations in traditional recombinant protein expression.
• Overview of techniques for achieving uniform post-translational modifications.
• Highlighting the advancements in in vitro and in cellulo backbone editing.