Gene Expression Profile of Ovine Endometrial Luminal Epithelial Organoids under Hormone Stimulation

Embryo implantation is a critical event in mammalian reproduction, directly impacting livestock efficiency and human reproductive health. However, the molecular mechanisms governing hormonal regulation of endometrial receptivity remain poorly understood. To address this, our project utilizes ovine endometrial organoids as a novel in vitro model that closely mimics physiological conditions, offering an unprecedented window into uterine biology. We exposed these organoids to key reproductive hormones and applied high-throughput transcriptome sequencing to capture the dynamic gene expression landscape during hormonal stimulation. This enables us to reconstruct the molecular events underlying endometrial preparation. Our objectives are to: 1) identify core signaling pathways and critical genes through which hormones regulate receptivity; 2) discover novel biomarkers for endometrial health assessment and implantation outcome prediction; and 3) establish a reliable research platform for reproductive studies and therapeutic screening. This integrated approach advances fundamental knowledge in reproductive biology while offering substantial applications: enhancing breeding efficiency in livestock, providing new insights into implantation disorders in human medicine, and establishing new research paradigms in reproductive science.