Optic neuritis (ON) is a common manifestation of multiple sclerosis (MS), characterized by inflammation and demyelination of the optic nerves. Recent studies indicate increased expression of genes associated with stress granules (SGs) in MS, with T cell intracellular antigen 1 (TIA1) being a key component of SGs. However, the role of TIA1-mediated SGs in MS-ON remains unclear. In experimental autoimmune encephalomyelitis (EAE) mice, a model of MS, TIA1 and G3BP1+ (a cell marker of SGs) SGs were upregulated in retinal neurons and optic nerve astrocytes. Knockout of Tia1 in the CNS (Tia1Nestin-CKO mice) suppressed demyelination, inflammatory infiltration, and retinal ganglion cells (RGCs) loss in EAE mice. Mechanically, RNA-sequencing analysis revealed upregulation of cholesterol synthesis genes such as hmgcs1 in Tia1Nestin-CKO EAE mice. Furthermore, deletion of Tia1 in astrocytes (Tia1GFAP-CKO mice) also alleviated demyelination in optic nerves in EAE mice through decreasing SG formation and increasing HMGCS1 expression in Tia1-/- astrocytes by decreasing the sequestration of hmgcs1 mRNA into SGs. Treatment with diarylpropionitrile (DPN), an ERβ-ligand, partially restored demyelination in Tia1GFAP-CKO EAE mice. These findings uncover the role of TIA1-mediated SG dynamics in MS-ON, highlighting a novel mechanism and potential therapeutic target for treatment.
Fang et al. (Fri,) studied this question.