The growing global burden of diabetes warrants search for natural, food‐derived bioactive molecules, including peptides. In this paper, dipeptidyl peptidase 4 (DPP4), a type 2 diabetes drug target,inhibitory peptides from the seed protein hydrolysates of mung bean ( Vigna radiata ) were studied. The peptides were isolated using activity‐guided purification and identified by LC‐MS/MS. Two peptides: VSVPHKDAE (MPHPep1) and VAPPGKGEVR (MPHPep2) showed DPP4 inhibition via competitive and uncompetitive modes, respectively. The average K i value of MPHPep1 and MPHPep2 was found to be 1.40 mm and 0.23 mm, respectively. Both peptides were stable towards gastric digestion conditions. While MPHPep1 is stable towards intestinal digestion, MPHPep2 exhibited 64% loss in its activity. Moreover, the peptides are stable with DPP4 and retain near‐complete inhibition activity after incubation with DPP4 for 30 min. In vitro DPP4 inhibition observed in cell‐based assays using the Caco‐2 cell line further validated the inhibitory potential of these peptides. Finally, the possible interactions of MPHPep1 and MPHPep2 with DPP4 using molecular docking tool are discussed.
Srivastava et al. (Thu,) studied this question.