Abstract Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli , is a soilborne disease that causes severe losses in the common bean ( Phaseolus vulgaris L.) crop. The most effective control strategy is the use of resistant cultivars; however, few with high resistance are currently available. This study aimed to investigate the genetic control of resistance to Fusarium oxysporum f. sp. phaseoli and identify molecular markers associated with resistance alleles in the Mesoamerican cultivar BRS Esplendor. A total of 103 progenies from a cross between BRS Esplendor (resistant) and BRS Horizonte (susceptible) were evaluated in a naturally infested field in Santo Antônio de Goiás, Goiás, Brazil. The experiments were conducted in the winter seasons of 2018 and 2019, and plants were evaluated regarding response to Fusarium wilt. F 2 progenies were genotyped using the DArTseq technology, resulting in SNP and SilicoDArT markers. A linkage map was constructed, and genetic mapping was carried out to identify regions of interest. Finally, the markers obtained were validated. Genetic variability was observed for Fusarium wilt response, with high estimates of heritability (97%) and of genetic gains (48%). A linkage map was constructed with 2,189 markers, spanning 3,026 cM. Three QTLs associated with Fusarium wilt response were identified on chromosome PV08, accounting for 51% to 66% of phenotypic variation. These QTL intervals overlapped, indicating a single stable QTL across the different years. The hydrolysis probe (AN33KKZ) developed from this QTL exhibited 97% selection efficiency, proving to be an effective tool for carrying out marker-assisted selection (MAS).
Ciappina et al. (Tue,) studied this question.