Lignin–Carbohydrate Nano-Sized Structures: An Evidence of Intracellular Lignin Biosynthesis?

The spatial localization of plant secondary cell wall polymers is a controversial issue. A relief of parallel-organized cellulose microfibrils was discovered, on the surface of which spherical nanoparticles were visualized. Spherical nanoparticles with a diameter of 20-50 nm were isolated using size exclusion chromatography from an aqueous extract of differentiating xylem of Norway spruce and visualized by SEM and AFM. The composition of isolated nanoparticles was determined by pyrolytic GC-MS, 1H NMR spectroscopy, and nitrobenzene oxidation, followed by separation of the products by liquid chromatography. Lignin was detected in the isolated nanoparticles already at the stage of cell wall formation. The hypothesis about the intracellular synthesis of lignin was proposed based on the results obtained. Lignin in the form of a lignocarbohydrate complex is formed not in the cell wall, but inside the cell. The formation of lignin-carbohydrate complexes occurs in Golgi apparatus and vesicles, which discharged into the inner surface of the cell wall simultaneously with the deposition of cellulose microfibrils. A new model of the structure of secondary cell wall postulates the formation of cellulose microfibrils surrounded by lignin-carbohydrate spherical complexes having a carbohydrate shell and an aromatic core.