Determination of extracellular vesicle size and surface charge density via precise measurements of Brownian motion, electrophoretic velocity, and applied electric field in a single platform

Key Points

• Size and surface charge density of extracellular vesicles were effectively quantified using Brownian motion and applied electric fields.
• The method provided precise measurements of electrophoretic velocity, demonstrating a reliable approach to vesicle analysis.
• This approach integrates multiple measurement techniques on a single platform, enhancing efficiency in evaluating vesicle properties.
• Findings have implications for advancements in biomarker detection and therapeutic applications using extracellular vesicles.